.

They are given by Dr. Carol Ripollès Ávila and Ms. Maria Martínez Garcia, in the laboratory V0-160. Attendance is limited to 26 people per group. Attendance of the three sessions is required. Different rapid methods are assayed.

GUIDE (download PDF)

Mornings group

Wednesday, 21st November · 9:15 a.m.-12 noon

  • Sample preparation and plating automated procedures: dilutor Dilumat, homogenizers Stomacher, Pulsifier and Smasher, serial dilution systems Dilucup-Dilushaker and Serial Diluter; spiral platers WASP Touch y Eddy Jet 2
  • Rapid viable cell count methods: Vitroids and LENTICULE discs (certified reference microorganisms); Petrifilm and Petrifilm Aqua (plates and reader), DryPlates; Colilert-18, Pseudalert, Quanti-Tray; NEO-GRID (hydrophobic grid membrane); Milliflex Quantum; SimPlate; colony counter Flash & Go
  • Method for enumeration by miniaturized MPN: TEMPO AC and EC

Thursday, 22nd November – Thanksgiving day · 9:15-11:45 a.m.

  • Environmental control procedures: RODAC and Count-Tact plates; contact slides; Quick Swab; samplers MicroBio, TRIO.BAS DUO and Sampl’air; exclusive wipes premium; MonitorMark
  • Chromogenic culture media: SALMA One Day, ALOA, chromID Coli, Baird-Parker RPF agar, Brilliance Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Brilliance Staph 24 agar, Brilliance coliform agar, RAPID’Salmonella, RAPID’E.coli 2, RAPID’L.mono, rhamnose test, IRIS Salmonella agar, COMPASS Listeria agar, Chromocult coliform agar
  • Diagnostic kits: API (kit and reader), RAPIDEC, BBL Crystal ID (kit and reader), RapID ONE, Microbact, Microgen ID, EnteroPluri-Test
  • Other environmental control procedures:
    • Bioluminescence: MicroSnap
  • Immunological detection methods:
    • ELFA: VIDAS SPT
    • immunoprecipitation: 1-2 Test for Salmonella
    • lateral immunomigration: Singlepath, VIP Gold, Reveal 2.0 (kit and reader AccuScan Gold)
  • Method for enumeration by miniaturized MPN: reading of TEMPO AC and EC

Friday, 23rd November · 9-11 a.m.

  • Diagnostic kits: O·B·I·S·, enzyme confirmation cards, ID membranes
  • Other environmental control procedures:
    • ATP – bioluminescence: luminometers Clean-Trace, AccuPoint Advanced, MVP ICON and EnSURE
    • colorimetry and fluorimetry: SwabSURE, Contam Swab, FLASH, Clean Test, InSite, AllerSnap, FluoroSELECT
    • others: BioFinder
  • Immunological detection methods:
    • ELISA: allergen test kit (hazelnut)
    • confirmation by latex agglutination: Microgen latex, Oxoid latex test
    • lateral immunomigration: Reveal Q+ for DON (kit and reader AccuScan Gold), Reveal 3-D for allergens (milk), AllerFlow gluten
  • Molecular biology (alternatives to PCR): Molecular Detection System (MDS), Amplified Nucleic Single Temperature Reaction (ANSR)
  • Reading: rapid viable cell count methods, environmental control procedures, chromogenic culture media, diagnostic kits, immunological detection methods
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