.

They are given by Dr. Carol Ripollès Ávila and Ms. Maria Martínez Garcia, in the laboratory V0-160. Attendance is limited to 26 people per group. Attendance of the three sessions is required. Different rapid methods are assayed.

GUIDE (download PDF)

Mornings group

Wednesday, 21st November · 9:15 a.m.-12 noon

  • Sample preparation and plating automated procedures: dilutor Dilumat, homogenizers Stomacher, Pulsifier and Smasher, serial dilution systems Dilucup-Dilushaker and Serial Diluter; spiral plater Eddy Jet 2
  • Rapid viable cell count methodologies: Vitroids and LENTICULE discs (certified reference microorganisms); Petrifilm and Petrifilm Aqua (plates and reader), DryPlates; Colilert-18, Enterolert-E, Pseudalert, Quanti-Tray; NEO-GRID (hydrophobic grid membrane); Milliflex Quantum; SimPlate; colony counter Flash & Go

Thursday, 22nd November – Thanksgiving day · 9:15-11:45 a.m.

  • Environmental control procedures: RODAC and Count-Tact plates; Hycheck and other contact slides; Quick Swab; samplers MicroBio, TRIO.BAS DUO and Sampl’air; MonitorMark
  • Description and preparation of chromogenic culture media: SALMA One Day, ALOA, chromID Coli, Baird-Parker RPF agar, Brilliance Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Brilliance coliform agar, RAPID’Salmonella, RAPID’E.coli 2, RAPID’L.mono, rhamnose test, IRIS Salmonella agar, COMPASS Listeria agar, BBL CHROMagar Salmonella, BBL CHROMagar O157, BBL CHROMagar Staph aureus, Chromocult coliform agar, ID membranes
  • Miniaturization: TEMPO AC and EC (enumeration by miniaturized MPN)
  • Preparation of diagnostic kits: API (kits and reader), RAPIDEC, BBL Crystal ID (kits and reader), RapID ONE, Microbact, Microgen ID, EnteroPluri-Test
  • Preparation of immunological detection methods:
    • ELFA: VIDAS Easy SLM
    • immunoprecipitation: 1-2 Test for Salmonella

Friday, 23rd November · 9-11 a.m.

  • Preparation of diagnostic kits: O·B·I·S·, enzyme confirmation cards
  • Reading: rapid viable cell count methodologies, environmental control procedures, chromogenic culture media, enumeration by miniaturized MPN, diagnostic kits
  • Preparation and reading of other environmental control procedures:
    • ATP – bioluminescence: luminometers Clean-Trace and AccuPoint Advanced
    • colorimetry: SwabSURE, Contam Swab, FLASH, Clean Test, InSite, AllerSnap
    • others: BioFinder
  • Preparation and/or reading of immunological detection methods:
    • ELFA: VIDAS Easy SLM
    • confirmation by latex agglutination: Microgen latex, Oxoid latex test
    • immunoprecipitation: 1-2 Test for Salmonella
    • lateral immunomigration: Singlepath, VIP Gold, Reveal 2.0 and Reveal Q+ for DON (kits and reader AccuScan Gold), Reveal 3-D for allergens (milk), Ochratoxin-A rapid test, Gluten flow through, AllerFlow gluten
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