They are given by Dr. Artur Xavier Roig SaguésMs. Carol Ripollès Ávila and Ms. Maria Martínez Garcia, in the laboratory V0-160. Attendance is limited to 26 people per group. Attendance of the three sessions is required. Different rapid methods are assayed.

GUIDE (download PDF)

Mornings group

Wednesday, 21st November · 9:15 a.m.-12 noon

  • Sample preparation and plating automated procedures: dilutors DiluFlow Pro and Diluwel, homogenizers Pulsifier, BagMixer 400 SW and Mixwel, serial dilution systems Dilucup-Dilushaker and Serial Diluter; spiral platers easySpiral Dilute and WASP Touch
  • Rapid viable cell count methodologies: Vitroids, LENTICULE discs and Epower (certified reference microorganisms); Petrifilm and Petrifilm Aqua (plates and reader), DryPlates; Colilert-18, Enterolert-E, Pseudalert, Quanti-Tray; NEO-GRID (hydrophobic grid membrane); Milliflex Quantum; SimPlate; Soleris 32; colony counter Protos3

Thursday, 22nd November – Thanksgiving day · 9:15-11:45 a.m.

  • Environmental control procedures: RODAC, Click & Safe and Count-Tact plates; Hycheck and other contact slides; Quick Swab; samplers MicroBio, TRIO.BAS DUO and Sampl’air; MonitorMark
  • Description and preparation of chromogenic culture media: ASAP, ALOA, chromID Coli, Baird-Parker RPF agar, Brilliance Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Brilliance coliform agar, RAPID’Salmonella, RAPID’E.coli 2, RAPID’L.mono, rhamnose test, IRIS Salmonella agar, COMPASS Listeria agar, BBL CHROMagar Salmonella, BBL CHROMagar O157, BBL CHROMagar Staph aureus, CCA agar, Chromocult coliform agar, ID membranes
  • Miniaturization: TEMPO EC (enumeration by miniaturized MPN)
  • Preparation of diagnostic kits: API (kits and reader), BBL Crystal ID (kits and reader), RapID ONE, Microbact, Microgen ID, EnteroPluri-Test
  • Preparation of immunological detection methods:
    • immunoprecipitation: 1-2 Test for Salmonella

Friday, 23rd November · 9-11 a.m.

  • Preparation of diagnostic kits: O·B·I·S·, enzyme confirmation cards
  • Reading: rapid viable cell count methodologies, environmental control procedures, chromogenic culture media, enumeration by miniaturized MPN, diagnostic kits
  • Preparation and reading of other environmental control procedures:
    • ATP – bioluminescence: luminometers Clean-Trace and AccuPoint Advanced
    • colorimetry: SwabSURE, Contam Swab, FLASH, Clean Test, InSite, AllerSnap
    • others: BioFinder
  • Preparation and/or reading of immunological detection methods:
    • confirmation by latex agglutination: Microgen latex, Oxoid latex test
    • immunoprecipitation: 1-2 Test for Salmonella
    • lateral immunomigration: Singlepath, VIP Gold, Reveal 2.0 and Reveal Q+ for DON (kits and reader AccuScan Gold), Reveal 3-D for allergens (milk), Ochratoxin-A rapid test, Gluten flow through, Agitest for aflatoxins, Agitest for allergens (egg), AllerFlow gluten
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