Tuesday 26th

8:45-9:30 a.m. Handout material delivery

9:30-10:15 a.m. Official opening of the workshop

10:15-11:35 a.m. Opening lecture by Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona, Bellaterra -Cerdanyola del Vallès-, Barcelona):

  • Visión general de los métodos rápidos y miniaturizados, y la automatización en microbiología [Overview on rapid methods, automation and miniaturization in microbiology]:
    • Sampling of solid and liquid foods, surfaces, and air
    • Miniaturization: microtiter plate, most probable number (MPN), viable cell counts. Diagnostic kits
    • Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescence filter technique (DEFT), turbidity
    • Methods for viable cell counts, based on electric impedance and conductance
    • Methods for viable cell counts, based on ATP – bioluminiscence and colorimetry
    • Immunological methods for microbial and toxin detection and identification: immunomagnetic separation, ELISA and ELFA, lateral immunomigration, immunoprecipitation, reversed passive latex agglutination
    • Molecular biology methods for microbial detection and identification: hybridization (DNA and rRNA probes); polymerase chain reaction (PCR); DNA and rRNA characterization (fingerprinting, riboprinting); sequencing; proteomics

11:35-11.50 a.m. Breakfast

11:50 a.m.-1:10 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona, Bellaterra -Cerdanyola del Vallès-, Barcelona):

  • La polymerase chain reaction (PCR) y la secuenciación genómica masiva aplicadas a la seguridad alimentaria [Polymerase chain reaction (PCR) and massive genome sequencing applied to food safety]

1:10-2:40 p.m. Lunch

2:40-5:35 p.m. Microbiology companies · exhibition (Pasteur, Yersin, Evans and Margulis groups):

  • Molecular detection methods using real-time PCR for yeast and mold, pathogenic bacteria, and viruses in food (BIOTECON Diagnostics, GmbH)
  • QFast: integrated platform for detection of pathogens, DNA, toxins and allergens (iMiCROQ, SL)
  • Importance of environmental monitoring in food industry and its effect on GFSI schemes (Intertek Ibérica Spain, SLU)
  • Process automation in industrial microbiology. Air samplers, dilutors, homogenizers, spiral plater, automatic colony counter and lateral flow test readers (IUL, SA)

5:35-5:50 p.m. Break

5:50-6:30 p.m. Ms. Paloma Bellver Moreira (Laboratorio de Saúde Pública de Galicia, Lugo):

  • Incidencia de la implantación del cribado, molecular o inmunológico, para investigar patógenos alimentarios y ambientales. Casos prácticos [Incidence of molecular or immunological screening implementation for food and environmental pathogen research. Practical cases]

6:30 p.m. Merienda

Wednesday, 27th November

Campus d'excel·lència internacional U A B