Tuesday 20th

9-9:45 a.m. Handout material delivery

9:45-11:35 a.m. Official opening of the workshop. Opening lecture by Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona, Bellaterra -Cerdanyola del Vallès-, Barcelona):

  • "Visión general de los métodos rápidos y miniaturizados, y la automatización en microbiología" ["Overview on rapid methods, automation and miniaturization in microbiology"]:
    • Sampling and sample preparation of solid and liquid foods, surfaces, and air
    • Miniaturization: microtiter plate, most probable number (MPN), viable cell counts. Diagnostic kits
    • Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescence filter technique (DEFT)
    • Other methods to detect microorganisms: Fung’s double tube, Fung-Yu tube
    • Methods for viable cell counts, based on electric impedance and conductance
    • Methods for viable cell counts, based on ATP – bioluminiscence and colorimetry
    • Immunological methods for microbial and toxin detection and identification: immunomagnetic separation, ELISA and ELFA, reversed passive latex agglutination, lateral immunomigration, immunoprecipitation
    • Molecular biology methods for microbial detection and identification: hybridization (DNA and rRNA probes); polymerase chain reaction (PCR); DNA and rRNA characterization (fingerprinting, riboprinting); biosensors, biochips and microchips; proteomics

11:35-11:50 a.m. Breakfast

11:50 a.m.-1:10 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona, Bellaterra -Cerdanyola del Vallès-, Barcelona):

  • "La polymerase chain reaction (PCR) y la secuenciación genómica masiva aplicadas a la seguridad alimentaria" ["Polymerase chain reaction (PCR) and massive genome sequencing applied to food safety"]

1:10-2:40 p.m. Lunch

2:40-5:35 p.m. Microbiology companies · exhibition (Pasteur, Yersin, Evans and Margulis groups):

  • Molecular detection methods using real-time PCR for yeast and mold, pathogenic bacteria, and viruses in food (BIOTECON Diagnostics, GmbH)
  • QFast: integrated platform for pathogen detection (iMiCROQ, SL)
  • Application of ATP for industrial quality control (MicroPlanet Laboratorios, SL)
  • Molecular solutions in agri-food sector: DNA automated extraction in water and food matrixes; and qPCR detection of pathogenic microorganisms, GMOs and food fraud (Promega Biotech Ibérica, SL)

5:35-5:50 p.m. Break

5:50-6:30 p.m. Mr. Pablo de Vicente López (Asociación Española de Normalización y Certificación -AENOR-, Madrid):

  • "Proceso de validación y certificación ISO 16140 para métodos rápidos de microbiología" ["ISO 16140 validation and certification of rapid microbiological methods"]

6:30 p.m. Merienda

Wednesday, 21st November

Campus d'excel·lència internacional U A B