9-9:45 a.m. Handout material delivery
9:45-10:15 p.m. Official opening of the workshop
10:15-11:35 a.m. Opening lecture by Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona, Bellaterra -Cerdanyola del Vallès-, Barcelona)
- Overview on rapid methods, automation and miniaturization in microbiology
- Sampling of solid and liquid foods, surfaces, and air
- Miniaturization: microtiter plate, most probable number (MPN), viable cell counts. Diagnostic kits
- Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescence filter technique (DEFT), turbidity
- Methods for viable cell counts, based on electric impedance and conductance
- Methods for viable cell counts, based on ATP – bioluminiscence and colorimetry
- Immunological methods for microbial and toxin detection and identification: immunomagnetic separation, ELISA and ELFA, reversed passive latex agglutination, lateral immunomigration, immunoprecipitation
- Molecular biology methods for microbial detection and identification: hybridization (DNA and rRNA probes), polymerase chain reaction (PCR), DNA and rRNA characterization (fingerprinting, riboprinting), sequencing, proteomics
11:35-11:50 a.m. Breakfast
11:50 a.m.-1:10 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona, Bellaterra -Cerdanyola del Vallès-, Barcelona):
- Polymerase chain reaction (PCR) and massive genome sequencing applied to food safety
1:10-2:40 p.m. Lunch
2:40-5:35 p.m. Microbiology companies · exhibition (Yersin group):
- How to detect and quantify coliphages according to the new European directives and national transpositions? (Bluephage)
- MALDI Biotyper in food microbiology. Rapid pathogen confirmation under ISO certification (Bruker Española)
- Condagene: quick pathogen detection by qPCR (Condalab)
- Work optimization in microbiology laboratories: from sample preparation to analysis (Interscience)
5:35-5:45 p.m. Break
5:45-6:30 p.m. Ms. Eva Tolosa Muñoz (Catalan Health Department, Subdirecció General de Seguretat Alimentària i Protecció de la Salut, Barcelona):
- Microbiological surveillance programmes and evaluation of results. Practical cases on alert management
6:30 p.m. Merienda
